Fig. 5: seRNAs in mixed cultures and organoids.
From: Selectively expressed RNA molecules as a versatile tool for functionalized cell targeting
a Growth of patterned U87 cell clusters (red circles) in the surrounded monolayer of primary neurons with subsequent transfer of seRNAΔ3-5−eGFP and seRNA-eGFP. For better visualization, zoom-in images are contrast-enhanced. b Transfer of the same seRNA constructs into neuronal networks and U87 tumor-spheres after growth for 5 days. n = 2 independent experiments. c Single transfer of seRNA-eGFP and seRNA-ca-Caspase3 into U87 target cells 24 h after seeding during tumor-sphere formation. Spheres are visualized in comparison to untreated U87 cells in phase contrast. Caspase activity was characterized by flow cytometry. Enhanced fluorescence at 450 nm corresponds to reduced viability. n = 4 independent experiments. d Repetitive delivery of seRNA-eGFP and seRNA-ca-Caspase3 into young U87 tumor spheres at days 2, 4, and 6 in the presence of BrdU. Subsequent cell proliferation was analyzed at indicated time points. All values are given with s.d. Scale bar = 200 µm. n = 2 to 3 independent experiments.
