Fig. 3: Single fiber proteomics in winter and summer muscles.
From: Reduced ATP turnover during hibernation in relaxed skeletal muscle
a Number of identified proteins per fiber (mean ± SEM; n = 4 bears for summer 1, winter 2; n = 5 for winter 1, n = 6 bears for summer 2) (b) MYH isoform distribution analysis of isolated single fibers from different bears over four different seasons (orange summer, blue winter). Each bar represents the relative abundance of MYH isoforms in one fiber in relation to the total sum of intensities for MYH1 (blue), MYH2 (red), MYH4 (purple), and MYH7 (green). Fibers from different bears are sorted into the four seasons and ranked from left to right of highest MYH2 abundance. c volcano plot for Type 2 A Fibers summer vs winter (n = 41–38). Differentially abundant proteins are highlighted in light blue (Welch’s t-test: FDR < 0.05, s0 > 0.1) d examples of proteins going up in summer (left) or winter (right). e changes in GO terms comparing summer and winter type 2 A fibers. The box plot represents the upper (75%) and lower (25%) whiskers scores outside the middle 50% with outliers from 0, 100% f Volcano plot for mixed (type1/2 A) Fibers summer vs winter (n = 20-30). Differentially abundant proteins are highlighted in light blue (Welch’s t-test: FDR < 0.05, s0 > 0.1). g Principal component analysis of protein expression patterns from isolated skinned single fibers of Type 2A (triangle) and mixed fibers (circle) collected in winter (blue) and summer (orange).
