Fig. 7: SA3K/SA3 protect CMs by inhibiting the WNT pathway and activating cardioprotective RISK and SAFE pathways.
From: Serpina3k lactylation protects from cardiac ischemia reperfusion injury
a–d Western blots (a, c) and quantification (b, d) of p-AKT, AKT, p-ERK, and ERK in neonatal mouse (a, b) and adult human CMs (c, d) treated with conditioned media from FBs with SA3K/SA3 overexpression or depletion. GAPDH was used as loading control. n = 4 (b) or 5 (d) independent experiments. e–h Western blots (e, g) and quantification (f, h) of p-JAK2, JAK2, p-STAT3 (Tyr705), and STAT3 in neonatal mouse (e, f) and adult human CMs (g, h) treated with conditioned media from FBs with SA3K/SA3 overexpression or depletion. GAPDH was used as loading control. n = 5 independent experiments. All overexpression data were analyzed using the two-tailed Mann-Whitney test, while all knockdown data were analyzed using the Kruskal-Wallis test followed by Dunn’s multiple comparisons. For (a, c, g), GAPDH loading control was run on a separate gel. Data are means ± SD. Source data are provided as a Source Data file.
