Fig. 3: Lung mesothelium is the source of transferred matrix in injury.
From: Targeting pleuro-alveolar junctions reverses lung fibrosis in mice
a Schematic representation of genetic pleural collagen protein fate mapping in animals. Mesothelial cells were transduced by intra-pleural injection of AAV particles expressing collagen reporters that monitor mesothelial-specific deposition of collagen. b Representative histology images of mouse lungs three days post-Col1-reporter injection. n = 6 biological (C57BL/6J WT mice) and 6 independent experiments replicates per reporter construct. Scale bars: 500 µm (Overview); 50 µm (high magnification). c Schematic, graphic and representative histology images of mouse lungs 10 and 14 days p.b.i. n = 6 biological (C57BL/6J WT mice) and 6 independent experiments replicates per reporter construct. Scale bars: 1000 µm (Overview); 50 µm (high magnification). Data represented are mean ± SD. A two-sided independent T-test was used for the comparison of two groups (Col1 reporter: p = 1.09e-07, Col2 reporter: p = 1.87e-07, CNA35: p = 4.49e-08)(***P < 0.001). d Schematic representation of the mesothelial-specific fate mapping of collagen protein in bleomycin-induced lung fibrosis. Upon oropharyngeal bleomycin installation pleural matrix pools that include newly deposited collagen (yellow) invade lung interstitium.
