Fig. 6: KLF5 regulates BRD4 recruitment to chromatin.
From: KLF5 loss sensitizes cells to ATR inhibition and is synthetic lethal with ARID1A deficiency
a Western blot analysis of chromatin-bound BRD4 long and short isoforms in WT and KLF5 KO U2-OS cells (Top). Histone H3 was used as marker for chromatin-bound fraction. And whole-cell extracts (bottom). *- cross-reacting protein species. Representative of at least 3 independent experiments. b Genome browser views of KLF5 CUT&Tag (CnT) (upper two panels), BRD4 CnT (middle two panels), and the negative control using IgG antibody (lower panel) in WT (grey peaks) and KLF5 KO (red peaks) U2-OS cells. c Metagene (upper panel) and Heat map (lower panel) demonstrating changes in BRD4 chromatin binding upon KLF5 loss. BRD4 binding sites were called based on WT relative to KLF5 KO cells, (n = 3). d Western blot analysis showing BRD4 levels upon siRNA depletion in WT and KLF5 KO U2-OS cells. GAPDH was used as a loading control. *- cross-reacting protein species. L: Long, S: Short forms of BRD4 protein. Representative of at least 3 independent experiments. e Clonogenic survivals of WT and KLF5 KO5 U2-OS ± BRD4 siRNA depletion treated with ATRi. Error bars = means ± SEM (biological n = 3). Data represented as AUCs. Statistical analyses were performed by one-way ANOVA with multiple comparisons. Source data are provided as a Source Data file.
