Fig. 7: The short isoform of NHSL3 recruits 14-3-3θ to lamellipodia.
From: NHSL3 controls single and collective cell migration through two distinct mechanisms
hTERT-HME1 cells are transfected with siRNAs targeting all NHSL3 forms (a) or specifically long and short isoforms (b). Cells are subjected to immunofluorescence with DAPI, phalloidin and antibodies recognising 14-3-3θ or cortactin. Enrichment of 14-3-3θ and cortactin at the lamellipodial edge is measured and plotted as the fold increase compared with signal intensity of a similar region of interest beneath the plasma membrane, mean ± SEM. Scale bars: 10 µm. n = 57 cells for (a) and n = 60 cells for (b). Statistical significance is calculated with two-sided Mann-Whitney test (a) or Kruskal-Wallis test (b) and p-values are indicated. Three biological repeats of both experiments gave similar results. Source data are provided as a Source Data file.
