Fig. 5: Repeat fungal exposure increases the proportion of cDC2s in the lung and dLN.
From: Mgl2+ cDC2s coordinate fungal allergic airway type 2, but not type 17, inflammation in mice
Representative tSNE plots reveal DC subsets identified by unbiased clustering analysis of flow cytometry data from the (A) lung and (B) dLNs of mice repeatedly exposed intranasally to PBS or Af spores (4 × 105 per dose). Graphs display the numbers and percentage of DC subsets isolated from the (A) lung and (B) dLNs. Data are from 2 independent experiments (n = 35 biologically independent animals). Data were fit to a linear mixed effect model, with experimental day as a random effect variable, and groups compared with a two-sided Tukey’s multiple comparison test. *p < 0.05, **p < 0.01, ***p < 0.001. Data are presented as mean values ± SEM. A P Values, cDC1; PBS vs Af d12 = 3.53 × 10−7, PBS vs Af d19 = 0.000142. CCR7+ DC; PBS vs Af d12 = 5.422 x 10-7, PBS vs Af d19 = 0.000142. cDC2 Mgl2+CD209a+; PBS vs Af d12 = 2.07 × 10−5, PBS vs Af d19 = 1.35  10−6. cDC2 Mgl2-CD209a+; PBS vs Af d12 = 1.08 × 10−5, PBS vs Af d19 = 0.0139. cDC2 Mgl2+CD209a-; PBS vs Af d12 = 4.05 × 10−5, PBS vs Af d19 = 1.02 × 10−5. Inf DC2; PBS vs Af d12 = 4.32 × 10−5. Inf DC3; PBS vs Af d12 = 7.25 × 10−5, PBS vs Af d19 = 0.00422. Monocyte 1; PBS vs Af d12 = 3.02 × 10−6. Monocyte 2; PBS vs Af d12 = 2.29 × 10−8, PBS vs Af d19 = 1.89 × 10−5. CD11b+; PBS vs Af d12 = 6.57 × 10−7, PBS vs Af d19 = 0.00209. B P Values, CCR7+ DC; PBS vs Af d12 = 0.00668. cDC2 Mgl2+CD209a+; PBS vs Af d12 = 2.07 × 10−5, PBS vs Af d19 = 1.35 × 10−6. cDC2 Mgl2+CD209a-; PBS vs Af d12 = 0.00512. Inf DC1; PBS vs Af d12 = 0.000319. Inf DC2; PBS vs Af d12 = 0.00841. Monocyte 1; PBS vs Af d12 = 0.0234. CD11b+; PBS vs Af d12 = 0.023. MHCIIlo; PBS vs Af d12 = 0.014. Source data are provided as a Source Data File.
