Fig. 6: Mgl2 cDC2s are crucial for promoting fungal type 2 allergic airway inflammation.

A WT or Mgl2-DTR mice were treated with diphtheria toxin (DTx) (on d6 and d9) whilst being exposed to repeat doses of Af spores (4 ×10⁵ per dose) BAL fluid and lung tissue were harvested 24 h after the final dose. Created in BioRender. Cook, P. (2024) https://BioRender.com/z24b189. B Representative flow cytometry plots identifying MΦ subsets and graphs show the number of MΦs in the lung tissue. C, D Representative flow cytometry tSNE plots show unbiased clustering analysis of identified subsets within the DC population that were detected in the dLN. Graphs show the number and percentage of DC subsets in the (C) lung and (D) dLN. E Graphs show the number and percentage of macrophages (MΦs), eosinophils (Eos), neutrophils (Neut) and lymphocytes (Lymph) detected in the BAL fluid 24 h after the sixth dose. F Representative flow cytometry plots identify IL-13⁺ and IL-17⁺ populations via intracellular cytokine staining of lung CD4⁺ T cells post stimulation with PMA/ionomycin. Graphs show the proportion of CD4⁺ T cells expressing type 1, type 2 and type 17 cytokine. B, E data from 3 independent experiments (n = 29 biologically independent animals). C, D data from 2 independent experiments (n = 18 biologically independent animals). F data from 4 independent experiments (n = 41 biologically independent animals). Data were fit to a linear mixed effect model, with experimental day as a random effect variable, and groups compared with a two-sided Tukey’s multiple comparison test. *p < 0.05, **p < 0.01, ***p < 0.001. Data are presented as mean values ± SEM. B P values, Alv MΦ; WT vs Mgl2-DTR = 0.00403. C P values, CCR7⁺ DC; WT vs Mgl2-DTR = 0.0158. cDC2 Mgl2⁺CD209a⁺; WT vs Mgl2-DTR = 0.00021. cDC2 Mgl2^-CD209a⁺; WT vs Mgl2-DTR = 0.00845. cDC2 Mgl2⁺CD209a^-; WT vs Mgl2-DTR = 0.00311. Inf DC2; WT vs Mgl2-DTR = 0.027. Inf DC3; WT vs Mgl2-DTR = 0.00783. CD11b⁺; WT vs Mgl2-DTR = 0.0399. D P values, cDC2 Mgl2⁺CD209a⁺; WT vs Mgl2-DTR = 1.62 × 10⁻¹⁴. cDC2 Mgl2⁺CD209a^-; WT vs Mgl2-DTR = 0.0332. E P values, Total Cells; WT vs Mgl2-DTR = 0.000503. Eos; WT vs Mgl2-DTR = 0.000149. Neut; WT vs Mgl2-DTR = 0.0131. F P values, IL-4; WT vs Mgl2-DTR = 0.000773. IL-5; WT vs Mgl2-DTR = 7.69 × 10^-5. IL-13; WT vs Mgl2-DTR = 0.000636. Source data are provided as a Source Data File. Schematics in figures were created in https://BioRender.com.