Fig. 1: Overall architecture of yCPT1.
a 3.2 Å resolution cryo-EM density map of dimeric yCPT1. Each ligand, motif, and assembly are colored according to the panel legend: yellow, catalytic assembly; purple, CDP-AP signature motif; gray, dimerization assembly; brown, N-terminal stretch; cyan, phosphatidylcholine (PC); blue, diacylglycerol (DAG). Positions of lumenal and cytoplasmic membranes shown in top panel. b Model of dimeric yCPT1. Atomic models corresponding to the density in panel a. In one protomer of the model each ligand, motif, and assembly are colored similarly as in panel a. In the lumenal view, the TM helix is numbered and the juxtamembrane helix (JMH) is labeled. Inter-monomer PCs and DAG are depicted as sticks. c Surface model of yCPT1 colored with hydrophobicity index by Chimera50. DAG is shown as balls within the surface model colored in blue(carbon) and red(oxygen). The cross-section plane depicted in panel d is also shown. d Cross-section of yCPT1 at the substrate-binding pocket. CDP-choline-binding site is enclosed in a solid red line. The color code of the models and the hydrophobicity index of yCPT1 is the same as shown in the panel c.
