Fig. 3: Local disorder of methylation in DNMT3A^INS AML occurs predominantly at bivalent domains.

a Average histone modification levels around hyper-DMR identified between DNMT3A^INS and normal bone marrow cells. Shades denote standard errors. b ChromHMM chromatin context enrichment of hyper-DMR identified between DNMT3A^INS and normal bone marrow cells. c Average methylation level surrounding bivalent domains. d LPMD distribution across different genomic contexts for Li2016 cohort. Parenthesized values denote the proportion of the analyzed CpGs within each genomic context, except for that next to ‘All’, which denote the total number of analyzed CpGs. P-values from two-sided Mann–Whitney U tests between DNMT3A^WT and DNMT3A^INS are shown. LPMD distributions in (e) bivalent domain and (f) SINE. P values from two-sided Mann–Whitney U tests between DNMT3A^WT and DNMT3A^INS are shown. n.s. p > 0.05. g LPMD comparison in bivalent or non-bivalent promoters. P-values from two-sided Mann–Whitney U tests between DNMT3A^WT and DNMT3A^INS are shown. Throughout (d–g), the center line denotes the median, the upper and lower box limits denote upper and lower quartiles, and the whiskers denote 1.5× interquartile range.