Fig. 1: Initial scaffold development using a group IIB intron RNP.
From: Scaffold-enabled high-resolution cryo-EM structure determination of RNA
A The maps of the group IIB RNP were generated using a tilted data collection strategy with the stage set to 30°. The map on the left was globally refined and shown at both a high (teal) and low threshold (white). The group II intron RNP displays clear RNA features at high threshold and density for the embedded RNA is observed at low threshold. Performing local refinement on the density corresponding to the embedded RNA yielded the map on the left (gold) which has been overlaid on the low threshold globally refined map (white) for clarity. B A model for the embedded RNA was fit into the locally refined map. The fit shows that the overall fold of the embedded RNA was maintained. C The viewing direction distribution for the data set is shown. Even with a 30° stage tilt, there is still a preferred orientation for this specimen.
