Fig. 2: Identification of a high-resolution group IIC intron scaffold.
From: Scaffold-enabled high-resolution cryo-EM structure determination of RNA
A The secondary structure of the O.i. group IIC intron scaffold (teal) is shown highlighting domain III (gold). The G to A mutation in the active site is highlighted red. B Cryo-EM density is shown for the scaffold. Domain III (gold) forms a stable stem loop that extends away from the rest of the folded RNA. C A local resolution map shows that the core of the scaffold has a resolution of 2.4 Å. D The viewing direction distribution plot generated from cryoSPARC is shown. The plot is evenly populated indicating that the group IIC intron scaffold exhibits no preferred orientation.
