Fig. 6: In vivo transfection and biodistribution of hybrid lipoplexes in tumor model after intravenous injection. | Nature Communications

Fig. 6: In vivo transfection and biodistribution of hybrid lipoplexes in tumor model after intravenous injection.

From: The hybrid lipoplex induces cytoskeletal rearrangement via autophagy/RhoA signaling pathway for enhanced anticancer gene therapy

Fig. 6

a The pGL3 transfection of hybrid lipoplexes (RLS/HS@Au) in subcutaneous HepG2 xenograft mice, evaluated by bioluminescence imaging from an in vivo imaging system at 24 and 48 h after intraperitoneal administration of D-luciferin. n = 5 mice. b Representative luciferase expression in dissected organs of mice at 48 h after intravenous injection of different lipoplexes (20 μg pGL3/mouse). Representative images of in vivo c and ex vivo d biodistribution of indicated lipoplexes (containing 20 μg Cy5-DNA) in tumor-bearing nude mice at 2, 4, 8, and 24 h after intravenous injection. n = 3 mice. The red circle indicates the location of the tumors. e Bioluminescence quantification in major organs after administration of saline, RLS/HS/pGL3, and RLS/HS@Au/pGL3, respectively. n = 5 mice. f Fluorescent intensity of major organs at 24 h after intravenous injection of saline, naked plasmid DNA, RLS/HS, and RLS/HS@Au containing Cy5-labeled DNA. n = 3 mice. A two-sided unpaired Student’s t-test was used for the comparisons in (e) and (f). The data in (e and f) are mean ± SD. p values < 0.05 were considered statistically significant. Source data are provided as a Source Data file.

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