Fig. 2: Php5 forms a trimeric complex that localizes to heterochromatic repeats. | Nature Communications

Fig. 2: Php5 forms a trimeric complex that localizes to heterochromatic repeats.

From: PhpCNF-Y transcription factor infiltrates heterochromatin to generate cryptic intron-containing transcripts crucial for small RNA production

Fig. 2

a Php5 immunopurified fractions were prepared from untagged and Php5-GFP expressing cells and were probed with anti-GFP antibodies. b Immunopurified fractions from untagged and Php5-GFP expressing cells were subjected to mass spectrometry analysis. The percentage of total peptide coverage for the indicated immunopurified proteins is displayed. Results from one biological replicate are shown; see also Supplementary Fig. 2a. c, d ChIP-qPCR analysis of the indicated GFP-tagged TFs was performed at cenH (c) and dh (d) in wild-type cells. Data from 3 independent biological experiments are presented as the mean ± SD of the relative fold enrichment compared to the leu1 control locus. The p-values were calculated using a two-tailed paired t-test. e, f ChIP-seq analysis to determine the localization of the indicated GFP-tagged TFs at the silent mat locus (e) and centromere 1 (f) in wild-type cells. Note that the Php5-GFP ChIP-seq data is the same as in Fig. 1a, b, as the experiments were performed simultaneously. g, h Euler diagrams represent the number of loci bound by Php5, Php3, and Php2 and contain a high (g) or low (h) abundance of CCAAT boxes. i, j Heat maps displaying ChIP-seq enrichments of PhpC subunits at loci containing high (i) or low (j) abundance of CCAAT boxes. Source Data are provided as a Source data file.

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