Fig. 4: The histone fold domain of PhpC is pivotal for heterochromatin localization.
a, b ChIP-seq analysis of Php3 and Moc3 localization at the silent mat locus (a) and centromere 1 (b) in wild type, clr4∆, and nda3-KM311 strains. WT Moc3-GFP and Php3-GFP ChIP-seq data are replotted from Figs. 1a, b and 2e, f. Vertical yellow lines designate CCAAT boxes. Note that the highlighted CCAAT-enriched region is accessible to Php3 in clr4∆ cells. The small black line denotes the dg-annealing primer used in Supplementary Fig. 4c. c, d ChIP-qPCR analysis of GFP-tagged Php3 or Moc3 was performed at cenH (c) and dh (d) in the indicated strains. e, f ChIP-seq analysis of Php3 localization at the silent mat locus (e) and centromere 1 (f) in wild type and php3L12R strains. g ChIP-qPCR analysis of GFP-tagged Php3 was performed at cenH and dh in indicated strains. h Representative live-cell images of strains expressing GFP-tagged Php3 or Php3L12R from two independent experiments with similar results. Data from 3 independent biological experiments are presented as the mean ± SD. The p-values were calculated using a two-tailed paired t-test (c, d, g). Source data are provided as a Source Data file.
