Fig. 5: Cooperative function of PhpC and Moc3 promotes cenH transcription. | Nature Communications

Fig. 5: Cooperative function of PhpC and Moc3 promotes cenH transcription.

From: PhpCNF-Y transcription factor infiltrates heterochromatin to generate cryptic intron-containing transcripts crucial for small RNA production

Fig. 5

a RT-PCR was performed to analyze cenH transcripts in the indicated strains, with leu1 serving as the control for +RT and -RT reactions. The primer binding site at cenH is indicated by the black line. b RT-qPCR analysis of cenH transcripts in the indicated strains. Relative expression was compared to the leu1 control locus. c RT-PCR was performed to analyze cenH top and bottom strand transcripts in the indicated strains, with leu1 used as the control for +RT and -RT reactions. The top and bottom strand-specific primer binding site at cenH is indicated by the red and yellow lines, respectively. Representative results from two independent experiments are shown. d RNA-seq expression profile of the cenH top and bottom strand in the indicated strains. Reads were uniquely mapped to cenH. e, f RNA polymerase II (8WG16) occupancy at cenH was determined by ChIP-seq (e) and ChIP-qPCR (f) analyses in the indicated strains. Reads were uniquely mapped to cenH. For f, ChIP data is presented as relative fold enrichment compared to the tRNA control locus. Data from 3 independent biological experiments are presented as the mean ± SD. The p-values were calculated using a two-tailed paired t-test (b, f). Source data are provided as a Source Data file.

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