Fig. 7: PhpC contributes to de novo heterochromatin establishment. | Nature Communications

Fig. 7: PhpC contributes to de novo heterochromatin establishment.

From: PhpCNF-Y transcription factor infiltrates heterochromatin to generate cryptic intron-containing transcripts crucial for small RNA production

Fig. 7

a 10-fold serial dilution assay of wild-type (WT) and CCAATmut strains on the indicated medium before and after trichostatin A (TSA) treatment and washout. Iodine staining was performed on non-selective (N/S) plates. b ChIP-qPCR analysis of H3K9me3 enrichment at the silent mat locus in the indicated TSA-treated strains. Data is presented as the mean of 3 independent biological experiments. Numbered lines in the schematic indicate the location of the primers. c Representative live-cell images of WT and CCAATmut strains harboring the mat2P::GFP reporter at the indicated time points (generations) after TSA treatment and washout. d Quantification of the cell fraction in the “ON” state (e.g., GFP-positive) at the indicated time points is shown. N = 260–651 cells for each data point. Source data are provided as a Source Data file.

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