Fig. 3: Macrophages, monocytes and neutrophils recruited in the omentum after B. abortus infection upregulate PD-L1 and Sca-1.

a Normalized absolute numbers of peritoneal LPM, SPM, monocytes, and neutrophils expressing PDL1, Sca-1, or both markers at 8 and 30 days post-infection, obtained by flow cytometry from mice treated as in Fig. 2a are shown. Each symbol represents one animal. (PBS (black circle), 2-3 mice; Ba (red square), 3-4 mice), n = 3. Mean from pooled data ± SD. Significant differences are shown (Mann-Whitney test, two-sided). Absence of p-value, non-significant. b Normalized absolute numbers of peritoneal LPM, SPM, monocytes, and neutrophils expressing total LAG-3 at 8 days p.i. obtained by spectral cytometry from mice infected as in (a) are shown. Each symbol represents one animal. (PBS (black circle), 3-4 mice; Ba (red square), 4−5 mice), n = 3. Mean ± SD. Significant differences are shown (Mann-Whitney test, two-sided). c Normalized absolute numbers of omental LPM, SPM, monocytes, and neutrophils expressing PD-L1, Sca-1, or both markers or expressing LAG-3 only or LAG-3 with or without PD-L1 at 8 days p.i. obtained by spectral cytometry from mice infected as in (a) are shown. Each symbol represents one animal. (PBS (black circle), 2-3 mice; Ba (red square), 4 mice), n = 2. Mean ± SD. Significant differences are shown (Mann-Whitney test, two-sided). Absence of p-value, non-significant. Source data are provided as a Source Data file.