Fig. 5: Metagenome sequencing reveals diet-specific delays in microbiome recovery in mice fed HF/HS diet on day 15. | Nature Communications

Fig. 5: Metagenome sequencing reveals diet-specific delays in microbiome recovery in mice fed HF/HS diet on day 15.

From: Klebsiella oxytoca facilitates microbiome recovery via antibiotic degradation and restores colonization resistance in a diet-dependent manner

Fig. 5

a Schematic showing the experimental setup. All mice were switched to one of four diets on day 0. Two days later, two groups on each diet began ampicillin treatment while one group per diet was left untreated. On day 5, one ampicillin-treated group was colonized with K. oxytoca while the other ampicillin-treated group received no bacterial inoculation. Fecal samples were collected for metagenome sequencing on 15 dpd. b PCoA plot calculated by Bray-Curtis distances of annotated KEGG functional modules in fecal samples collected on day 15 from mice either untreated, ampicillin treated or ampicillin treated and K. oxytoca colonized from one experiment with n = 3–4 mice/group. c, d Analysis of differentially abundant (c) bacterial taxa (from de novo generated MAG profiles) and (d) carbohydrate-active enzymes (CAZymes) in mice that show K. pneumoniae clearance versus mice that show no clearance by LEfSe. Panel (a) Created with BioRender.com.

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