Fig. 2: Differential accumulation and chloroplast localization of Tig1 and Tig2. | Nature Communications

Fig. 2: Differential accumulation and chloroplast localization of Tig1 and Tig2.

From: A truncated variant of the ribosome-associated trigger factor specifically contributes to plant chloroplast ribosome biogenesis

Fig. 2

a Representative confocal microscopy images of Tig1 and b Tig2 accumulation in chloroplasts of Arabidopsis. GFP signal is shown in green, chlorophyll autofluorescence is shown in magenta. An additional overview of Tig2-GFP is presented in Supplementary Fig. 4. Experiments were performed in two biological replicates with two independent lines. c Heatmap illustrating transcript changes during heat (22 °C to 32 °C), cold (22 °C to 4 °C) and highlight (80 to 450 μmol photons m-2 s-1) acclimation and de-acclimation. Transcript values were extracted from24 and are given as log2 fold change of Fragment Per Kilobase Million (FPKM) relative to the initial time point. d Quantitative immunoblot of Tig1 and Tig2 from leaf tissue of Col-0 grown for 4 weeks at 21 °C or plants, which were exposed for 1 weak at 21 °C and 3 weeks at 4 °C. Data are means from three biological replicates, recombinant protein served as standard, error bars represent standard deviations (SD).

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