Fig. 1: Treg are less effective in regulating allogeneic responses mediated by HP1α-deficient T cells than by their wild-type counterparts. | Nature Communications

Fig. 1: Treg are less effective in regulating allogeneic responses mediated by HP1α-deficient T cells than by their wild-type counterparts.

From: Heterochromatic gene silencing controls CD4+ T cell susceptibility to regulatory T cell-mediated suppression in a murine allograft model

Fig. 1

Lethally-irradiated B6 mice were grafted with a 1:1 mixture of B6 (H-2b) and B6D2F1 (H-2bd) bone marrow and co-injected with WT or HP1α-deficient naive CD4+ T cells alone or in the presence of ex vivo expanded WT Treg. To control engraftment, a group of mice was also injected solely with the bone marrow cell mixture. a Experimental model. b Representative dot-plots showing the frequency of syngeneic (H-2Kb+) and semi-allogeneic (H-2Kbd+) cells in the blood 21 days after engraftment. c Percentage of semi-allogeneic (H-2Kd+) cells among peripheral blood mononuclear cells (PBMC) 21 days after engraftment. Data show mean ± SEM of 3 to 6 independent experiments. Each symbol represents the mean of an experiment. P values were calculated using multiple unpaired t-tests with Holm-Šidák correction. Source data are provided in the Source data file.

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