Fig. 2: HP1α-deficient Th1 and Th17 cells are poorly repressed by Treg.

Lethally-irradiated B6 mice were grafted with a 1:1 mixture of B6 and B6D2F1 bone marrow and co-injected with WT or HP1α-deficient naive CD4⁺ T cells alone or in the presence of ex vivo expanded WT Treg. a Representative dot-plots showing 21 days after engraftment the percentage of WT and HP1α KO TCR Vβ6⁺CD4⁺ T cells producing IFN-γ and IL-17A. b–d Percentage of WT or HP1α KO TCR Vβ6⁺CD4⁺ T cells producing IFN-γ (b), IL-17A (c) or both (d) 21 days after engraftment. e Volcano plot showing results of differential gene expression analyses between Treg that had been co-injected with WT or HP1α KO naive CD4⁺ T cells. Red and black dots represent genes with higher expression in Treg co-injected with HP1α KO or WT cells, respectively. Gray dots represent genes that failed to reach the FDR threshold of 0.05 and the absolute log2 fold change threshold of 1. f Absolute number of spleen cells 21 days after engraftment. Data are mean ± SD of three (Tconv) or seven (Tconv + Treg) biological replicates from two independent experiments. g Percentage of Tconv, defined as CD4⁺CD45.1^-CD45.2⁺H-2K^d-Thy1.1^-, in the spleen 21 days after engraftment. Data are mean ± SEM of three independent experiments. h Percentage of allospecific Tconv, defined as CD4⁺CD45.1^-CD45.2⁺H-2K^d-Thy1.1^-TCRVβ6⁺, in the spleen 21 days after engraftment. Data are mean ± SEM of three independent experiments. i, j Percentage of CD73⁺FR4⁺ (i) or Foxp3⁺ (j) cells among WT or HP1α KO TCR Vβ6⁺CD4⁺ T cells, as determined in the spleen 21 days after engraftment. b–d and g–j Data show mean ± SEM of 3 independent experiments. Each symbol represents the mean of an experiment. P values were calculated using multiple unpaired t test with Holm-Šidák correction. Source data are provided in the Source data file.