Fig. 5: Th celI inhibition and conversion into anergic or regulatory T cells is more efficient in the absence of HP1γ.

Lethally-irradiated B6 mice were grafted with a 1:1 mixture of B6 and B6D2F1 bone marrow and co-injected with WT (a-d and I, j) or HP1γ-deficient (a–j) naive CD4⁺ T cells alone or in the presence of ex vivo expanded WT Treg. a Representative dot-plots showing 21 days after engraftment the percentage of WT and HP1γ KO TCR Vβ6⁺ Tconv producing IFN-γ and IL-17A. b, c Percentage of WT or HP1γ KO TCR Vβ6⁺ Tconv producing IFN-γ (b) or IL-17A (c) 21 days after engraftment. d Percentage of CD73⁺FR4⁺ cells among WT or HP1γ KO TCR Vβ6⁺ Tconv, as determined in the spleen 21 days after engraftment. e Percentage of cytokine-producing cells among HP1γ KO TCR Vβ6⁺ Tconv 21 days post-engraftment. The analysis was performed on total Tconv from mice injected with Tconv only, or on anergic (A) or non-anergic (N-A) Tconv from mice injected with Tconv and Treg. (f) Representative dot-plots showing 21 days after engraftment the percentage of HP1γ KO TCR Vβ6⁺ Tconv expressing PD-1 and TIGIT. g, h Percentage of PD-1⁺, TIGIT⁺, LAG-3⁺ (g) or PD-1⁺TIGIT⁺LAG-3⁺ (h) cells among HP1γ KO TCR Vβ6⁺ Tconv 21 days after engraftment. i Percentage of Foxp3⁺ cells among WT or HP1γ KO TCR Vβ6⁺ Tconv, as determined in the spleen 21 days after engraftment. j Percentage of spleen Foxp3⁺ cells among anergic or non-anergic WT and HP1γ KO TCR Vβ6⁺ Tconv 21 days after engraftment. b–d and I, j Data are represented as mean ± SEM of three independent experiments. Each symbol represents the mean of an experiment. P values were calculated using multiple unpaired t test with Holm-Šidák correction. e, g, h Floating bar charts represent the mean of the biological replicates as well as the minimum and maximum values. Each symbol represents individual biological replicates. P values were calculated using unpaired t test (two-tailed). Source data are provided in the Source data file.