Fig. 6: HP1γ negatively controls immune checkpoint expression in effector Th cells.

a Pattern of expression of genes overexpressed in Treg-exposed HP1γ KO Tconv compared with WT Tconv. (b) Cluster 2 genes expression in indicated Tconv populations. Horizontal bars represent the median, and the top and bottom of the box the upper and lower quartile, respectively. The whiskers go from the minimum to the lower quartile and from the upper quartile to the maximum. Statistical significance was calculated using the Pairwise Wilcoxon Rank Sum Test (two-tailed). c Exhaustion signature enrichment analyses of cluster 2 genes. d GO enrichment analyses of cluster 2 genes. e Euler diagram showing the relationship between cluster 2 genes and those associated with peaks more open in Treg-exposed HP1γ KO Tconv than WT Tconv. Exhaustion signature enrichment analysis was run on genes common to both groups. f ATAC–seq and RNA-seq tracks at the Lag3 locus. g ATAC-seq signal at peaks associated with cluster 2 genes. Peaks were divided into two sets according to whether or not they were differentially open in WT and KO T cells exposed to Treg. h–k PD-1 and LAG3 expression on naive CD4⁺ T cells before or after two days of culture with anti-CD3 antibody. h, j Representative histograms showing the expression of PD-1 (h) or LAG3 (j) in naive and activated T cells. i, k Percentage of activated T cells expressing PD-1 (i) or LAG3 (k) (left) and average immune checkpoint expression per cell (right). l Representative dot-plots showing PD-1 versus LAG3 expression by ex vivo stimulated CD4⁺ T cells with or without (control) DMSO or chaetocin. m, n Percentage of CD4⁺ T cells, either ex vivo activated and treated or not (control) with DMSO or chaetocin, expressing PD-1 (m) or LAG-3 (n). I, k, m, n Data show mean ± SD of biological replicates from 4 independent experiments. Each symbol represents an individual biological replicate. P values were calculated using unpaired t test (two-tailed). Source data are provided in the Source data file.