Fig. 7: HP1γ-deficient human CD4+ T cells are more susceptible to Treg-mediated suppression than wild-type cells. | Nature Communications

Fig. 7: HP1γ-deficient human CD4+ T cells are more susceptible to Treg-mediated suppression than wild-type cells.

From: Heterochromatic gene silencing controls CD4+ T cell susceptibility to regulatory T cell-mediated suppression in a murine allograft model

Fig. 7

HLA-B7- WT or HP1γ-deficient human naive CD4+ T cells were injected i.v. into sublethally-irradiated NSG mice with or without ex vivo expanded HLA-B7+ human Treg. Three weeks after injection, splenocytes were isolated and the xenogeneic T cell response was analyzed by flow cytometry. a Experimental model. b, c Following genome editing by CRISPR-Cas9, the expression level of HP1γ was determined in WT and HP1γ KO human CD4+ T cells. A representative western-blot (b) and normalized expression levels for each experiment (c) are shown. d Representative dot-plots showing the percentage of HLA-B7- WT and HP1γ KO human Tconv producing IFN-γ and GM-CSF. e–g Percentage of HLA-B7- WT and HP1γ KO human Tconv producing IFN-γ (e), GM-CSF (f) or Granzyme B (g). h Expression level of T-bet in HLA-B7- WT and HP1γ KO human CD4+ T cells. i, j Percentage of HLA-B7- WT and HP1γ KO human CD4+ T cells producing IL-10 (i) or expressing Foxp3 (j). Data show values for biological replicates from two (e–j) or three (c) independent experiments. Horizontal bars represent mean ± SD (e–j) or mean ± SEM (c). P values were calculated using two-tailed unpaired t tests. Source data are provided in the Source data file.

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