Fig. 3: Localization of the gonadal gdf6Y expression in XY animals. | Nature Communications

Fig. 3: Localization of the gonadal gdf6Y expression in XY animals.

From: The master male sex determinant Gdf6Y of the turquoise killifish arose through allelic neofunctionalization

Fig. 3

Transcripts of gdf6Y (magenta) and the GC marker ddx4 (green) as well as the (a) Sertoli or (b) somatic supporting cell marker amh (orange) were detected in N. furzeri testes at (a) sexually mature (n = 9; here: 3 months old) and (b) 0 dph stage (n = 7). a, b White arrows – cells co-expressing gdf6Y and amh. c Minimal distances of gdf6Y signals to ddx4 and amh signals from 4 XY gonads at 0 dph including (b). Left: Violin plots of minimal distances <8 µm (n = 212 per gene, equals lowest 95%) with median, quartiles, and respective modes (bin width: 0.1 µm) from the 4 individual gonads (circle, square, triangle, and diamond). Statistical testing by a two-tailed Mann–Whitney test. Right: Frequency distribution of all measured minimal distances (bin width: 0.1 µm, n = 224). Source data are provided as a Source Data file.

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