Fig. 4: Oogenesis starts before 0 dph, depends on foxl2l, and can be measured and intercepted by genetic modification in N. furzeri. | Nature Communications

Fig. 4: Oogenesis starts before 0 dph, depends on foxl2l, and can be measured and intercepted by genetic modification in N. furzeri.

From: The master male sex determinant Gdf6Y of the turquoise killifish arose through allelic neofunctionalization

Fig. 4

a Volcano plots of DEGs (FDR < 0.05) between XX and XY animals in the whole embryo at 10 dpf and in trunk parts at 0 and 3 dph as derived from a respective previously published data set (XX at 0 dph n = 2, others n = 3; Supplementary Data 1)8. b Overlap of female DEGs between 0 and 3 dph. c Overrepresentation analysis (ORA) of KEGG pathways within the overlap of female DEGs between 0 and 3 dph. d Schematic of CRISPR/Cas9-based chimeric mutant (CRISPant) assay to address the influence of a goi (gene of interest) on oogenesis onset as a proxy for sexual differentiation. T7EI – T7 endonuclease I. Created in BioRender. Richter, A. (2024) https://BioRender.com/t02m033. e Expression of female marker genes on mRNA-level in trunks of females (XX; n = 8), phenofemales (GRZ-gdf6Ydel9 XY*; n = 4), and males (XY; tacc3, n = 5; zp4, n = 6) and their mosaic foxl2l− counterparts (XX, n = 6; XY*, n = 5; XY, n = 3) at 0 dph. Welch’s ANOVA and Dunnett’s T3 multiple comparisons test with XX animals. P-values < 0.05 are displayed. c, e Source data are provided as a Source Data file.

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