Fig. 1: mosGILT is expressed in the mosquito midgut and upregulated in response to a blood meal.
From: mosGILT antibodies interfere with Plasmodium sporogony in Anopheles gambiae
a Immunoblots of A. gambiae midgut (MG) and salivary glands (SG) probed with rabbit mosGILT polyclonal antibodies at a 1:10000 dilution. b Immunostaining of the anterior (upper panels) and posterior (lower panels) MG regions using purified mosGILT mouse polyclonal antibodies (0.8 μg/μl) at a 1:500 dilution (red). Alexa Fluor 647 goat anti-mouse IgG served as the secondary at a 1:1000 dilution. Blue: DAPI staining of the nuclei. Ovalbumin (OVA) purified mouse polyclonal antibody served as a negative control. c RT-qPCR analysis of mosGILT expression levels in midguts from sugar-fed mosquitoes (control, n = 12) and midguts collected 1 (p = 0.5369, n = 16), 3 (p = 0.0014, n = 15), 5 (n = 14), and 7 (p = 0.5604, n = 14) days post-blood meal. Each dot represents an individual mosquito midgut, with the thick horizontal lines indicating medians and thin lines denoting upper and lower quartiles. Two experimental replicates were performed. Two-Tailed Mann-Whitney was used to determine significance (ns: insignificant, **p ≤ 0.01, ****p ≤ 0.0001). Source data are provided as a Source Data file.
