Fig. 5: Active immunization with mosGILT decreases P. berghei oocyst number and infection prevalence.

a Experiment scheme for C57BL/6 female mice injected with recombinant mosGILT (mosGILT) or control protein (OVA), and boosted twice, at two-week intervals. b Two weeks after the final boost, blood samples were taken from the mice. Sera were examined for mosGILT-specific antibodies by ELISA. Mice with strong titers were challenged with P. berghei-infected red blood cells (iRBC). Mosquitoes blood fed on the mice 5 or 6 days after Pb infection. c Number of P. berghei oocysts per midgut and infection prevalence (pie charts) of mosquitoes that took a blood meal from a P. berghei-infected mouse actively immunized with mosGILT (n = 118) or control (n = 72) protein. Oocysts were counted seven days post-infected blood meal. Each dot represents the number of oocysts in an individual mosquito midgut, and the blue and red horizontal lines indicate the medians for the control and intervention groups, respectively. The thin lines indicate upper and lower quartiles. Three experimental replicates were performed. Two-Tailed Mann-Whitney was used to determine the significance of oocyst abundance. Fisher’s exact test was used to compare infection prevalence values (****p ≤ 0.0001). Source data are provided as a Source Data file.