Fig. 7: MosGILT antibodies function in the midgut and decrease the expression of the ecdysone receptor (EcR) and lipophorin (Lp).

a Summary of the experimental scheme of a partial immunized blood meal followed by infected blood meal (IBM). b Number of P. berghei oocysts from mosquitoes that took a partial blood meal on a GST (Control + IBM; n = 35), or mosGILT (mosGILT + IBM, n = 29) antisera immunized mouse followed by an IBM 48 h later (p = 0.0060). c Number of P. berghei oocysts from mosquitoes that engorged on an IBM 48 h after intrathoracic injection with 1.4 μg of purified mosGILT (n = 38) or control (n = 31) antibodies (p = 0.9688). Oocysts were counted eight days post-IBM. The EcR (d) and Lp (e) expression level in midguts 48 h after engorgement on animals immunized with mosGILT (n = 12) or control (n = 12) antibodies, as determined by RT-qPCR (d, p = 0.0007; e, p = 0.0449). The expression levels of EcR (f) and Lp (g) 48 h after a blood meal on a mouse before (control; n = 16) or after retro-orbital injection with mosGILT antibodies (n = 16), as determined by RT-qPCR (f, p = 0.0058; g, p = 0.0387). Each dot represents an individual mosquito midgut, and the blue and red horizontal lines indicate the medians for the control and intervention groups, respectively. The thin lines indicate upper and lower quartiles. At least wo experimental replicates were performed for each experiment. Two-Tailed Mann-Whitney was used to determine significance (ns: not significant, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001). Source data are provided as a Source Data file.