Fig. 6: Recurrence and pulmonary metastasis of 4T1-luc tumors.

a Schematic illustration of the tumor recurrence study. b–e Tumor growth profiles (b), representative bio-luminescent images (c), tumor weights (d), and images of TUNEL-stained tumor sections (e) of mice bearing 4T1-luc tumors after different treatments (n = 6 mice). Scale bars = 50 μm. f Schematic illustration of the design of the pulmonary metastasis inhibition experiment. g–i Changes in the intensities of bio-illuminance signals (g), bio-illuminance images of mice at day 42 (h), and survival curve (i) of mice bearing 4T1-luc tumors after different treatments (n = 8 mice). j, k Numbers of lung metastasis (j) and representative images of H&E-stained lung sections (k) from mice bearing 4T1-luc tumors at day 28 after different treatments (n = 4 mice). Scale bars = 500 μm. Data were presented as the mean ± SD and the statistical significance was calculated with one-way ANOVA following by the Tukey’s tests for tumor weights and numbers of lung metastasis. Two-way ANOVA following by the Tukey’s tests was used to analyze the tumor growth in the recurrence model and the survival curve was statistically analyzed with the log-rank tests. MST median survival time, H&E hematoxylin and eosin, TUNEL terminal deoxynucleotidyl transferase-mediated dUTP nick-end-labeling. Source data are provided as a Source Data file.