Fig. 5: IFNs preferentially upregulate HLA-B expression in ECN90 β-cells. | Nature Communications

Fig. 5: IFNs preferentially upregulate HLA-B expression in ECN90 β-cells.

From: Interferon-α promotes HLA-B-restricted presentation of conventional and alternative antigens in human pancreatic β-cells

Fig. 5

a Relative mRNA expression (mean + SEM of 11 biological replicates) of HLA-A, HLA-B, and HLA-C genes in ECN90 β-cells exposed to the indicated IFNs for 24 h. GAPDH was used as an internal normalizing control, and each gene was normalized to the basal sample; p-values by Mann-Whitney U test indicated on top of bars. b, c Protein expression of HLA-A, -B and -C in ECN90 β-cells exposed to the indicated IFNs, as detected by surface flow cytometry (b; mean + SEM of 17 biological replicates) and Western blot (c; representative experiment out of 3 performed) using the indicated Abs validated for their specificity (see Supplementary Fig. 6a, b). For flow cytometry, p-values by the Mann-Whitney U test are indicated on top of the bars. For Western blotting, the arrowhead indicates the HLA-I heavy chain band, with the top band indicating the α-tubulin loading control and normalized HLA-I fold change (FC) values indicated. Additional analyses are presented in Supplementary Fig. 7.

Back to article page