Fig. 6: Experimental validation of therapeutic hypotheses. | Nature Communications

Fig. 6: Experimental validation of therapeutic hypotheses.

From: Real-world clinical multi-omics analyses reveal bifurcation of ER-independent and ER-dependent drug resistance to CDK4/6 inhibitors

Fig. 6

Dose response curves evaluating the viability of MCF7 and MCF7 ESR1 Y537S cells after exposure to Palbociclib (a) or Fulvestrant (b) at different concentrations for 7 days. Cells treated with DMSO were used as control for normalization. Data shown on the y-axis are mean ± SD (n = 3). P-values were calculated by one-tailed student’s t-test. c Western blot analysis was performed to determine the expression of CDK2 in the presence of 100 ng/ml Doxycycline. Cells were harvested at 4 days after the Doxycycline treatment and lysates were immunoblotted with the indicated antibodies. d Cell Colony formation assay was conducted in the 12-well plates in the absence or presence of 50 ng/ml Doxycycline. Crystal violet staining of cells was performed after 14 days of cell growth. Three shRNAs against CDK2 and one non-targeted shRNA as control were tested. e Quantitative analysis of cells colony formation by scanning the intensities of crystal violet stained cell colonies using LI-COR Odyssey CLx Imaging System. Data shown on the y-axis are mean ± SD (n = 12) of six replicates in two independent experiments. P-values were calculated by one-tailed Student’s t-test. Source data are provided as a Source Data file.

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