Fig. 3: Restoration of histomorphometric bone and bone marrow properties in iliac crest biopsies from myelofibrosis patients after allo-HSCT. | Nature Communications

Fig. 3: Restoration of histomorphometric bone and bone marrow properties in iliac crest biopsies from myelofibrosis patients after allo-HSCT.

From: Reconstructing skeletal homeostasis through allogeneic hematopoietic stem cell transplantation in myelofibrosis

Fig. 3

a Representative histological images of Gomori’s trichrome-stained sections in myelofibrosis patients before allo-HSCT (Pre-HSCT) and after allo-HSCT (Post-HSCT), along with the control group (Control). b Representative histological images of trichrome Masson-Goldner-stained sections of the three groups. c Representative histological images of von Kossa-stained sections of the three groups. d Fibrosis area to marrow area (Fb.Ar/Ma.Ar) of myelofibrosis patients before allo-HSCT (Pre, n = 20) and after allo-HSCT (Post, n = 20), along with the control group (Ctrl, n = 20). e Adipose area to marrow area (Ad.Ar/Ma.Ar) of the three groups (Pre, n = 20; Post, n = 20; Ctrl, n = 20). f Osteoid volume to bone volume (OV/BV) of the three groups (Pre, n = 20; Post, n = 20; Ctrl, n = 20). g Percentage of patients with osteomalacia (defined as OV/BV > 2%22) in each group (Pre, n = 20; Post, n = 20; Ctrl, n = 20). h Bone volume to tissue volume (BV/TV) of the three groups (Pre, n = 20; Post, n = 20; Ctrl, n = 20). i Mineralized volume to tissue volume (Md.V/TV) of the three groups (Pre, n = 20; Post, n = 20; Ctrl, n = 20). j Trabecular number (Tb.N) of the three groups (Pre, n = 20; Post, n = 20; Ctrl, n = 20). k Trabecular thickness (Tb.Th) of the three groups (Pre, n = 20; Post, n = 20; Ctrl, n = 20). The median (center line, 50th percentile) and interquartile range (box edges, 25th–75th percentile) with whiskers extending to the minimum and maximum values are depicted. Each data point is shown. For osteomalacia, the percentage of patients in each group is displayed. Differences between the three groups were calculated using one-way ANOVA with Holm-Šidák test (h, i) or Kruskal-Wallis H test with Dunn’s test (d–g, j, k) for normally and non-normally distributed data, respectively. Exact p-values of the comparisons are displayed above the brackets and numbers in bold indicate statistical significance (p < 0.05). Source data are provided as a Source Data file.

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