Fig. 1: NKp46 supports IL-18R1, IL-7Rα, and IL-2Rα expression on ILC1s. | Nature Communications

Fig. 1: NKp46 supports IL-18R1, IL-7Rα, and IL-2Rα expression on ILC1s.

From: NKp46 enhances type 1 innate lymphoid cell proliferation and function and anti-acute myeloid leukemia activity

Fig. 1

a Ncr1+/+ and Ncr1gfp/gfp ILC1s were sorted for RNA-seq from the liver of Ncr1+/+ mice and Ncr1gfp/gfp mice, respectively, using FACS. Volcano plots show statistically significant differentially expressed genes in RNA pools (Ncr1gfp/gfp ILC1s vs. Ncr1+/+ ILC1s) (n = 5). b–d Expression of IL-18R1 (b), IL-7Rα (c), and IL-2Rα (d) on the liver Ncr1+/+ ILC1s and Ncr1gfp/gfp ILC1s was measured by flow cytometry (n = 6). e Ncr1+/+ ILC1s and Ncr1gfp/gfp ILC1s were treated with IL-18 (10 ng/ml) at the indicated time points. Phosphorylation of P38 was measured by flow cytometry (Ncr1+/+: n = 5; Ncr1gfp/gfp: n = 4). f, g Ncr1+/+ ILC1s and Ncr1gfp/gfp ILC1s were treated with IL-7 (100 ng/ml; f) or IL-2 (1000 IU/ml; g) at the indicated time points. Phosphorylation of STAT5 was measured by flow cytometry (f: n = 7; g: n = 5). Data were presented as means ± s.d.; P values were calculated by either a two-tailed Student’s t-test (b–d and f at 30 min) or linear mixed models with adjustments (e, g). Source data are provided as a Source Data file.

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