Fig. 2: NKp46-triggered signaling activates the NF-κB signaling pathway and IL-2Rα expression.

a Hallmark pathway analysis in Ncr1^+/+ ILC1s and Ncr1^gfp/gfp ILC1s RNA pools (Ncr1^gfp/gfp ILC1s vs. Ncr1^+/+ ILC1s). The panel shows signaling pathways downregulated in Ncr1^gfp/gfp ILC1s. Genes with a false discovery rate (FDR)-adjusted P value < 0.05 and a fold change (FC) > 1.5 or < 0.7 were significantly upregulated or downregulated, respectively (n = 5). b Gene set enrichment analysis (GSEA) plots show enrichment of the TNF gene in the NF-κB signaling pathway in ILC1s. The x-axis shows the rank orders (Ncr1^gfp/gfp ILC1s vs. Ncr1^+/+ ILC1s) of all the targeted genes (n = 5). c, d Ncr1^+/+ ILC1s and Ncr1^gfp/gfp ILC1s were treated with or without anti-NKp46 antibody (5 μg/ml) for the indicated time. Phosphorylation of P65 (p-P65) was measured by flow cytometry. Representative histogram (c) and quantification of p-P65 mean fluorescence intensity (MFI) (d) in Ncr1^+/+ ILC1s and Ncr1^gfp/gfp ILC1s at the indicated time points (n = 9). FMO: fluorescence minus one. Data from two experiments were pooled. e Ncr1^+/+ ILC1s and Ncr1^gfp/gfp ILC1s were treated with anti-NKp46 antibody (5 μg/ml) in the presence or absence of NF-κB inhibitor (Triptolide, 10 nM) for 3 days. Representative histogram of (top) and quantification (bottom) of IL-2Rα MFI on Ncr1^+/+ ILC1s and Ncr1^gfp/gfp ILC1s (n = 14). f ILC1s were transfected with control silence RNA (siRNA-control) or siRNA (siRNA-1/2/3 mix) for interfering P65 for 3 days using lipofectamine 3000. Histogram (left) and statistics graphic (right) showing the expression of P65 in ILC1s (n = 7). g ILC1s were transfected with control siRNA or siRNA (siRNA-1/2/3 mix) for interfering P65 for 3 days using lipofectamine 3000. Histogram (left) and statistics graphic (right) showing the expression of IL-2Rα in ILC1s (n = 7). Data were presented as means ± s.d.; P values were calculated by linear mixed models with adjustments (d), one-way ANOVA models with adjustments (e), or a paired two-tailed Student’s t-test (f, g). NS not significant. Source data are provided as a Source Data file.