Fig. 4: Engagement of NKp46 expressed on ILC1s augments their effector function.

a Ncr1^+/+ ILC1s and Ncr1^gfp/gfp ILC1s sorted from the liver of Ncr1^+/+ and Ncr1^gfp/gfp mice, respectively, were cultured with or without anti-NKp46 antibody (5 μg/ml) for 12 h. Representative flow cytometry (left) and statistics (right) of the IFN-γ⁺ (top) and TNF⁺ (bottom) of ILC1s (n = 6). b Ncr1^+/+ ILC1s and Ncr1^gfp/gfp ILC1s sorted from the liver of Ncr1^+/+ and Ncr1^gfp/gfp mice, respectively, were cultured with or without C1498 AML cells expressing luciferase (C1498-Luc) for 12 h. Representative flow cytometry (left) and statistics (right) of the IFN-γ⁺ (top) and TNF⁺ (bottom) of ILC1s (n = 6). c Ncr1^+/+ ILC1s and Ncr1^gfp/gfp ILC1s sorted from the liver of Ncr1^+/+ and Ncr1^gfp/gfp mice, respectively, were cultured with C1498-Luc AML cells for 12 h. ILC1s were loaded into the bottom chambers of a 96-well Transwell plate. The top wells of the plate were loaded with C1498-Luc AML cells in the presence of IL-12 plus IL-15 cytokines. Statistics of the IFN-γ⁺ (left) and TNF⁺ (right) of ILC1s (n = 4). Created in BioRender. Ma, R. (2024) https://BioRender.com/a44t133. d Ncr1^+/+ ILC1s and Ncr1^gfp/gfp ILC1s sorted from the liver of Ncr1^+/+ and Ncr1^gfp/gfp mice, respectively, were cultured at the indicated ratios with C1498-Luc AML cells for 24 h. Luciferase activity in the wells with tumor cells was measured with a luminescence microplate reader (n = 6). Created in BioRender. Ma, R. (2024) https://BioRender.com/n74x942. Data were presented as means ± s.d. P values were calculated by either an unpaired Student’s t-test (a–c) or two-way ANOVA models with adjustments (d). NS not significant. Source data are provided as a Source Data file.