Fig. 1: DUX4 induction after herpesviral infection.

A Western blot of primary HFF cells infected with HSV-1 for 24 h and 293T cells infected with HSV-1 for 18 h, both at a MOI 5. ICP0 was used as marker for infection. Representative experiment out of n = 3. B RNA-seq. data of the DUX4 and neighboring FRG1 and FRG2 loci in WI38 9 hpi, HFF 4 hpi and HEK 293T cells 18 hpi. The Precision Run-On Sequencing (Pro-Seq) data show RNA pol II occupancy on the FRG1, FFRG2 and DUX4 locus in infected and uninfected cells. ICP4 ChIP-Seq data show ICP4 occupation at the DUX4 locus in cells infected with HSV-1. C Western blot of iSLK rKSHV 219 cells induced with 1 µg/ml Doxycycline for 1, 2 or 3 days. K8α run on a different gel and was used as control for KSHV reactivation. Representative experiment out of n = 3. D Western blot of 293T cells infected with HSV-1 for 18 h at a MOI of 5 and analyzed for expression of ZSCAN4. Glycoprotein D (gD) served as a control for viral infection. Representative experiment out of n = 3. E HFF cells infected with HCMV for 6 d with MOI 1. Western blot analysis of DUX4. HCMV glycoprotein B (gB) was used as marker for infection. Representative experiment out of n = 3. F RNA-seq. analysis of DUX4 target genes after HSV-1 or Ad5 infection. Ad5 RNA-seq. data was taken from BioProject PRJEB57806. Source Data are provided as a Source Data file.