Fig. 2: Herpesviral immediate-early proteins induce DUX4 expression.
From: Herpesviruses mimic zygotic genome activation to promote viral replication
A Western Blot of DUX4 and HSV-1 proteins ICP0, ICP27, VP16 and glycoprotein D (gD) in HFF cells infected with HSV-1 harvested at different hpi (MOI of 10). Representative experiment out of n = 3. B DUX4 expression kinetics of newly synthesized RNA (4su-sequencing) in HFF cells infected with HSV-1 wt and HSV-1 delta vhs virus. Reanalysis of data from Friedel et al.7. C Western Blot of DUX4 and HSV-1 proteins in primary HFF infected with HSV-1 and HSV-1 mutants (HSV-1 deltaICP0 (ICP4-YFP), HSV-1 deltaICP27, HSV-1 deltaICP34.5/ICP47) harvested at 16 hpi (MOI 10). One representative experiment out of n = 5. D Western blot analysis of DUX4 and HSV-1 protein in 293T cells transfected with HSV-1 IE proteins ICP0, ICP0 FXE, ICP4 (ICP4-YFP), ICP0 + ICP4 (ICP4-YFP) and ICP0 FXE + ICP4 (ICP4-YFP) for 48 h or infected with HSV-1 for 18 h (MOI of 10). ICP0 FXE is a mutant with a deletion in the RING domain, which inhibits Ubiquitin E3 ligase activity. EV: empty vector control. One representative experiment out of n = 3. E qRT-PCR analysis of cellular genes DUX4, TRIM43 as well as the viral genes ICP0 and gC in HDF-TERT cells untreated or treated with PAA and infected with HSV-1 (MOI of 0.1). Values are biological replicates and presented as mean fold induction +/-SD (normalized to HPRT RNA) relative to uninfected control cells. Source Data are provided as a Source Data file.
