Table 1 Reagents
From: Herpesviruses mimic zygotic genome activation to promote viral replication
Primary Antibodies and Plasmids | |||
|---|---|---|---|
Anti-DUX4 (E5-5) | Abcam | Ab124699 | |
Anti-DUX4 (clone 9A12) | EMD Millipore | MABD116 | |
Anti-DUX4 (P4H2) | Thermo Fisher Scientific | MA5-16147 | |
VP16 (14-5) | Santa Cruz Biotechnology Inc | Sc-7546 | |
HSV-1 ICP0 antibody (11060) | Santa Cruz Biotechnology Inc | Sc-53070 | |
HSV-1/2 ICP27 antibody | Santa Cruz Biotechnology Inc | Sc-69806 | |
HSV-1 gD antibody | Santa Cruz Biotechnology Inc | Sc-21719 | |
HSV-1 ICP4 (H943) antibody | Santa Cruz Biotechnology Inc | Sc-69809 | |
Anti-HA.11 | Covance | MMS-101R | |
THE GFP antibody | Genescript | A01704-40 | |
Anti-HCMV gB (27-287) | Hybridoma | ||
Anti-ZSCAN4 | Sigma-Aldrich/Merck Millipore | HPA006491 | |
THE β-Actin antibody, HRP | Genescript | A00730-40 | |
GAPDH antibody, Biotin | Genescript | A00915 | |
Β-Tubulin antibody | Genescript | A01717-40 | |
THE α-Tubulin antibody | Genescript | A01410-40 | |
Normal Rabbit IgG | Cell Signaling Technology | #2729 | |
HSP 90 (4F10) | Santa Cruz Biotechnology Inc | Sc-69703 | |
Plasmids | |||
LentiCRISPRv2 | Addgene | 52961 | |
pMD2.G | Addgene | 12259 | |
psPAX2 | Addgene | 12260 | |
pCW57.1-DUX4-CA | Addgene | 99281 | |
pCW57.1-Luciferase | Addgene | 99283 | |
pCW57.1_blasti | Addgene | 194067 | |
pCS2-mkgDUX4 | Addgene | 21156 | |
pTYB12 | NEB | N6709S | |
