Table 2 Oligonucleotides
From: Herpesviruses mimic zygotic genome activation to promote viral replication
Real time quantitative PCR probes | |||
|---|---|---|---|
DUX4L | 5’-TCAGCCAGAATTTCACGGAAG-3’ 5’-CCATTCTTTCCTGGGCATCC-3’ | FAM/ZEN/3IABκFQ | IDT |
ZSCAN4 | 5’-CTAGTCACACATCAGCTCAGT-3’ 5’-TTCAGTCTCTTGCCTTGTGTC-3’ | FAM/ZEN/3IABκFQ | IDT |
TRIM49B | 5’-GCTGCTGAGGAACACCA-3’ 5’-ATTGCTTCTAGCCTTAAATTCACAT-3’ | FAM/ZEN/3IABκFQ | IDT |
TRIM48 | 5’-ATCACTGGACTGAGGGACA-3’ 5’-GGGCGGATTTTGACGGT-3’ | FAM/ZEN/3IABκFQ | IDT |
ICP0 | 5’-CGGACACGGAACTGTTCGA-3’ 5’-CGCCCCCGCAACTGC-3’ | FAM/ZEN/3IABκFQ | IDT |
HPRT1 | 5’-CGAGATGTCATGAAGGAGATGG-3’ 5’-TCAGCAAAGAACTTATAGCCCC-3’ | HEX/ZEN/3IABκFQ | IDT |
VTRNA1 | 5’-TTTAATTGAAACAAGCAACCTGTCT-3’ | FAM/NFQ | Thermo Fisher Scientific |
TRIM43 | 5‘-TCATCTGATTCGACGGCAAG-3´ 5´-AGATGGTGACAGGGTCTACC-3´ | FAM/ZEN/3IABκFQ | IDT |
ICP27 | 5’-ACATCTTGCACCACGCCAG-3’ 5’-CGCCAAGAAAATTTCATCGAG-3’ | FAM/ZEN/3IABκFQ | IDT |
gG | 5’-GCAGGCAYACGTAACGCACGCT-3’ 5’-TTCTCGTTCCTCACTGCCTCCC-3’ | 5Cy5/ 3IAbRQSp | IDT |
BZLF1 | 5’-TTCAGAATCGCATTCCTCCAG-3’ 5’-CTAGCAGACATTGGTGTTCCA-3' | FAM/ZEN/3IABκFQ | IDT |
BRLF1 | 5’-CAGGAGTTAGCCTCAGAAAGTC-3’ 5’-ATACCATACAGGACACAACACC-3' | FAM/ZEN/3IABκFQ | IDT |
EBNA1 | 5’-CACATGTCGTCTTACACCATTGA-3’ 5’-TGTGGGCCGGGTCCA-3' | FAM/ZEN/3IABκFQ | IDT |
PCR primer for EMSA assay | |||
HSV1-BS1 | fwd: 5’-/5Cy5/ GTGTACCACTGCCACTGTCG-3’ rev: 5’-/5Cy5/ GTCTGATCATGCCCCATACC-3’ | IDT | |
HSV1-BS2 | fwd: 5’-/5Cy5/ ACATGACGTTCCACAGGTCC-3’ rev: 5’-/5Cy5/ TCACCGAGCCAGAAACTACG-3’ | IDT | |
HSV1-No-BS | fwd: 5’-/5Cy5/ CGTGAACCAAAGACGAGGGC-3’ rev: 5’-/5Cy5/ CCACGTTGAGAAGCTCGTCG-3’ | IDT | |
KSHV-BS1 | fwd: 5’-/5Cy5/ ACGTACTAAATCTACTTGACAC-3’ rev: 5’-/5Cy5/CAGACACTATCCCAGAAAC-3’ | IDT | |
KSHV-BS2 | fwd: 5’-/5Cy5/CTCTGTAGAGGTGATGTCC -3’ rev: 5’-/5Cy5/ATTATTATGAGGCCTATCTG -3' | IDT | |
KSHV-BS3 | fwd: 5’-/5Cy5/ATCTACATATAGCGTCTACACC-3’ rev: 5’-/5Cy5/AGGTTACATTAGTAGTGTCACG-3' | IDT | |
PCR primer for HSV-1 Standard | |||
HSV-1 gG | fwd: 5’-TTGGCACAAAAAGACCCCGA-3’ rev: 5’- AACTAGATACCACCGCCTTTATTG-3’ | IDT | |
PCR primer for nanobody cloning | |||
Nanobody | fwd: 5’- AGAGCTCGTTTAGTGAACCGTC-3’ rev: 5’- GGTGGACCGGTTCATTACTAAAC-3’ | IDT | |
Backbone | fwd: 5’- GTTTAGTAATGAACCGGTCCACC-3’ rev: 5’- GGCGATCTGACGGTTCACTAAAC-3’ | IDT | |
