Fig. 4: PRDX1 overexpression ameliorates NASH and liver fibrosis.

a Peroxidase activity of global hepatic PRDX in WT and Prdx1^OE/OE mice. WT mice (n = 5); Prdx1^OE/OE mice (n = 7). b Body weight of WT and Prdx1^OE/OE mice on WD. 8-week-old male mice were fed a WD and their body weights were monitored weekly. WT mice (n = 6); Prdx1^OE/OE mice (n = 5). c Daily food intake of mice on WD (as in b). WT mice (n = 6); Prdx1^OE/OE mice (n = 5). ns, no significance. d Energy expenditure (kcal) of mice on WD (as in b). WT mice (n = 6); Prdx1^OE/OE mice (n = 5). e Locomotion activity of mice on WD (as in b). WT mice (n = 6); Prdx1^OE/OE mice (n = 5). ns, no significance. f Fasting blood glucose levels of mice on WD (as in b). WT mice (n = 6); Prdx1^OE/OE mice (n = 5). g IPITT in mice on WD (as in b). WT mice (n = 6); Prdx1^OE/OE mice (n = 5). h Serum ALT and AST levels in mice on WD (as in b). WT mice (n = 6); Prdx1^OE/OE mice (n = 5). i Hepatic H₂O₂ levels in WD-fed mice (as in b). n = 5 mice per group. j Hepatic MDA levels in WD-fed WT and Prdx1^OE/OE mice (as in b). WT mice (n = 6); Prdx1^OE/OE mice (n = 5). k Representative images showing H&E and Oil Red O staining of liver after WD (as in b). n = 3 biologically independent mice. Scale bars, 50 μm. l Representative images from three mice per group showing Sirius Red and α-SMA staining of liver after WD (as in b). n = 3 biologically independent mice. Scale bars, 50 μm. m mRNA expression of hepatic genes after WD (as in b). WT mice (n = 6); Prdx1^OE/OE mice (n = 5). All the data are presented as means ± SEM. Unpaired and two-tailed Student’s t test was performed for a, c, d, e, f, h, i, j, and m. Two-way ANOVA followed by Bonferroni’s test for multiple comparisons was performed for b and g.