Fig. 6: PRDX1 protects liver mitochondrial function.
a Hepatic respiratory control ratio (RCR) of mice on NC or WD. 8-week-old male C57BL/6 mice were fed a NC or WD for 20 weeks before their liver mitochondria were isolated for O2k analyses. n = 3 mice per group. b Hepatic leaking control ratio (LCR) (as in a). n = 3 mice per group. c Hepatic citrate synthase activity (CSA) (as in a). n = 3 mice per group. d O2 flux in isolated liver mitochondria (as in a). n = 3 mice per group; ns, no significance. Mal, malate; Glu, glutamate; Suc, succinate; Cyt, cytochrome c; Ccc, cccp. ns, no significance by unpaired Student’s t test. e MDA concentration in the liver mitochondria isolated from mice after NC or WD (as in a). n = 3 mice per group. f Hepatic RCR of WT and Prdx1OE/OE mice. 8-week-old male WT and Prdx1OE/OE mice were fed a WD for 20 weeks before their liver mitochondria were isolated for O2k analyses. WT mice (n = 6); Prdx1OE/OE mice (n = 7). g Hepatic LCR (as in f). WT mice (n = 6); Prdx1OE/OE mice (n = 7). h Hepatic CSA (as in f). WT mice (n = 6); Prdx1OE/OE mice (n = 7). i O2 flux in isolated liver mitochondria (as in f). WT mice (n = 6); Prdx1OE/OE mice (n = 7). j MDA concentration in the liver mitochondria isolated from WT and Prdx1OE/OE mice after WD. n = 5 mice per group. All the data are presented as means ± SEM. Unpaired and two-tailed Student’s t test was performed for a–j.
