Fig. 3: Endothelial AGGF1 deficiency significantly ameliorated pathological neovascularisation in the OIR model.
From: Endothelial AGGF1 promotes retinal angiogenesis by coordinating TNFSF12/FN14 signalling
a–i FITC-dextran staining of whole-mount retinas and quantification of the avascular area and NVTs area from Aggf1fl/fl and Cdh5-Cre Aggf1fl/fl normoxic and OIR mice at P12 (a, d, g) (n = 10 Aggf1fl/fl and 8 Cdh5-Cre Aggf1fl/fl OIR mice), P17 (b, e, h) (n = 9 Aggf1fl/fl and 12 Cdh5-Cre Aggf1fl/fl OIR mice) and P21 (c, f, i) (n = 5 Aggf1fl/fl and 4 Cdh5-Cre Aggf1fl/fl OIR mice). In the inserts, the red line represents the edge of the retina, the blue area represents the avascular area, and the red area represents NVTs. j TER119-positive RBC leakage in Aggf1fl/fl and Cdh5-Cre Aggf1fl/fl OIR mice are shown (n = 5 mice per group). k F4/80-positive macrophage infiltration in Aggf1fl/fl and Cdh5-Cre Aggf1fl/fl OIR mice are shown (n = 5 mice per group). Error bars represent mean ± SEM. 2-tailed unpaired Student’s t test (g, h, i). Scale bars: 1000 μm (a–f) and 100 μm (j, k). Source data are provided as a Source Data file.
