Fig. 5: AGGF1 promoted the binding of TNFSF12 to Fn14 to increase angiogenesis.
From: Endothelial AGGF1 promotes retinal angiogenesis by coordinating TNFSF12/FN14 signalling
a AGGF1 protein interaction network analysed by STRING (string-db.org). b Immunoprecipitated (IP) AGGF1 was immunoblotted (IB) with AGGF1 or TNFSF12 antibody in HRMECs. c, d Western blot analysis and quantification of TNFSF12, CyclinA2, CyclinD1, and CDK1 protein levels in control and TNFSF12-silenced HRMECs treated with HG (n = 4 independent cultures). e, f Representative images and quantification of control and TNFSF12-silenced HRMECs treated with HG (Scratching wound healing assay) (n = 6 independent cultures). g Representative images of control and TNFSF12-silenced HRMECs treated with HG (EdU staining) (n = 3 independent cultures). h Western blot analysis of CyclinA2, CyclinD1, CDK1, and TNFSF12 protein levels in blank control and TNFSF12-plasmid HRMECs (n = 6 independent cultures). i Western blot analysis of CyclinA2, CyclinD1, CDK1, AGGF1, and TNFSF12 protein levels in blank control and AGGF1 + TNFSF12-plasmid HRMECs (n = 4 independent cultures). j Representative images of blank control and AGGF1 + TNFSF12-plasmid HRMECs (EdU staining) (n = 3 independent cultures). k TNFSF12 protein interaction network analysed by STRING (string-db.org). l Immunoprecipitated (IP) FN14 immunoblotted (IB) with AGGF1 or TNFSF12 antibody in HRMECs. m Immunoprecipitated (IP) FN14 immunoblotted (IB) with TNFSF12 or FN14 antibody in HRMECs treated with HG. n Schematic illustration of AGGF1 promoting the binding of TNFSF12 to Fn14 to increase angiogenesis. Error bars represent mean ± SEM. one-way ANOVA with Tukey’s multiple comparisons test (d, f). Scale bars: 1000 μm (e) and 100 μm (g, j). HG, high glucose (33.3 mmol/L). Source data are provided as a Source Data file.
