Fig. 6: Anti-AGGF1 treatment inhibits retinal angiogenesis.

a Schematic illustration of the OIR mice treated with intravitreal injections. At P13, mouse pups were intravitreally injected with 2.5 μL anti-IgG, anti-AGGF1, anti-TNFSF12, anti-AGGF1 + TNFSF12, anti-VEGF, or anti-AGGF1 + VEGF antibodies. Retinas were analysed at P17. b–d FITC-dextran staining of whole-mount retinas from OIR mice injected with anti-IgG (n  =  6 mice), or anti-AGGF1 antibody (n  =  5 mice), or anti-TNFSF12 antibody (n  =  4 mice), or anti-AGGF1 + TNFSF12 antibody (n  =  7 mice), or anti-VEGF antibody (n  =  6 mice), or anti-AGGF1 + VEGF antibody (n  =  11 mice). In the inserts, the red line represents the edge of the retina, the blue area represents the avascular area, and the red area represents NVTs. c, d Quantification of the avascular and NVT areas at P18 in OIR mice, related to B. Error bars represent mean ± SEM. one-way ANOVA with Tukey’s multiple comparisons test (c, d). Scale bars: 1000 μm (b). Source data are provided as a Source Data file.