Fig. 4: Homogeneous amplification and lossless index of MPHAC-DIS.

We encoded files of multimodal data, including text, pictures, etc., based on the encoding principle (Supplementary Fig. 26). a Simultaneous retrieval of 8 distinct files in the DNA pool using primer combinations. The top-to-bottom presentation includes original files, decoding results, and the distribution of sequence coverage, with red pixels indicating repeat reads lower than 5. Notably, the sequence coverage within each file is uniformly distributed, showcasing excellent intra-file homogeneity. b Coverage comparison between FE-primers and FL-primers. The results illustrate a high degree of consistency and highlighting the outstanding inter-file amplification homogeneity of MPHAC-DIS. c Fold-80 base penalty of FE-primers and FL-primers. Fold-80 base penalty is a metric used to assess coverage uniformity in sequencing experiments. It indicates how much more sequencing is needed for 80% of the targets to reach the mean coverage. The closer the Fold-80 base penalty is to 1, the better the coverage uniformity. d Correlation between the experimental ratios and the expected ratios of various files. Through multiple random-access experiments, we observed a linear relationship between the actual and expected ratio, providing additional confirmation of the inter-file amplification homogeneity of MPHAC-DIS. e Decoding accuracy comparison between FE primers and FL primers. The correct rate for the majority of files exceeds 99%, underscoring the exceptional performance of MPHAC-DIS. f Statistics on the frequency of sequence error types in MPHAC-DIS. Data points were obtained from the results of 30 libraries. Source data are provided as a Source Data file.