Table 1 Cryo-EM data collection, refinement, and validation statistics
From: Structure of transmembrane AMPA receptor regulatory protein subunit γ2
Data collection and processing | |
Magnification | 130,000x |
Voltage (kV) | 300 |
Electron exposure (e–/Å2) | 40 |
Defocus range (μm) | −1.0 to 2.6 |
Pixel size (Å) | 0.93 |
Symmetry imposed | C1 |
Initial particle images (no.) | 123,729 |
Final particle images (no.) | 494,916 |
Map resolution (Å) | 2.32 |
FSC = 0.143 | |
Map resolution range (Å) | 2–4 |
Refinement | |
Initial model used (PDB code) | N/A |
Model resolution (Å) | 2.3 |
FSC = 0.143 | |
Model resolution range (Å) | 2.1–3.7 |
Map sharpening B factor (Å2) | −54.8 |
Model composition | |
Non-hydrogen atoms Protein residues Ligands | 2678 172 0 |
B factors (Å2) | |
Protein Ligand | 0.00/27.91/5.8 N/A |
R.m.s. deviations | |
Bond lengths (Å) Bond angles (°) | 0.003 0.623 |
Validation | |
MolProbity score Clashscore Poor rotamers (%) | 1.23 1.5 0 |
Ramachandran plot | |
Favored (%) Allowed (%) Disallowed (%) | 95.12 4.88 0.00 |
