Fig. 2: Trim28 deficiency impairs CD8+ T cell activation in vitro. | Nature Communications

Fig. 2: Trim28 deficiency impairs CD8+ T cell activation in vitro.

From: TRIM28 is an essential regulator of three-dimensional chromatin state underpinning CD8+ T cell activation

Fig. 2

a Gene-expression heatmap for the RNA-seq data of TRIM family in murine naive and activated CD8+ T cells. b-e naive CD8+ T cells were cultured under anti-CD3/28 stimulation for 3 days. Expression of Trim28 was measured by qPCR (b). Expression of IFN-γ, TNF-α (c) and T-bet (d) was measured by flow cytometry. mRNA levels of Ifng, Gzmb, Tnfa, Il2ra, Il2 and Tbx21 were detected by qPCR (e). f Volcano plot of transcriptome differences between WT and Trim28-/- activated CD8+ T cells. g Top enriched GO pathways of genes regulated by TRIM28. h Heatmap of illustrating the relative expression of signature genes in WT and Trim28-/- activated CD8+ T cells. i GSEA enrichment of naive and activated CD8+ T cell signature genes in the transcriptome of CD8+ T cells from Trim28fl/flCd8aCremice. Each dot represents one individual replicate (n = 3 per group in b, c and e, n = 4 per group in (d). Error bars represent the SD. Statistical significance was tested by unpaired two-sided Student’s t-test (b–e). Data are representative of three independent experiments.

Back to article page