Fig. 6: Applications of eCas12f1 for base editing and regulation of gene expression.
From: Robust genome editing activity and the applications of enhanced miniature CRISPR-Cas12f1
a Constructs of adenine base editor with catalytically inactivated eCas12f1. TadA-TadA* was fused to the N-terminus of dead eCas12f1. TadA, wild type of TadA; TadA*, TadA-8e. b A-to-G conversion efficiency of eCas12f1-ABE at eight gene targets. c Construct of a cytosine base editor with catalytically inactivated eCas12f1. rAPOBEC and two copies of UGI were fused to the N- and C-termini of dead eCas12f1, respectively. d C-to-T conversion efficiency of eCas12f1-CBE in eight gene targets. e Construct of an eCas12f1 activator for activation of gene expression. VPR was fused to the C-terminus of dead eCas12f1. f Relative quantitation of mRNA level of three genes. The fold increase in gene expression is indicated above each bar graph. g Construct of an eCas12f1 inhibitor for interference with gene expression. DNMT3L and KRAB were fused to the N- and C-termini of dead eCas12f1, respectively. h Relative quantitation of mRNA level of three genes. Data represents mean ± s.d. of three independent biological replicates. P-values were obtained using the two-tailed Student’s t-test. Source data are provided as a Source Data file.
